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This study evaluated amylase production by Bacillus species employing the solid state fermentation (SSF) method using five agro-industrial wastes namely corn cobs, potato peel and maize straw, groundnut husk and corn chaff. Five Bacillus species were tested for amylase production abilities and Bacillus subtilis showed the highest amylase production ability after incubation. Corn chaff gave maximum enzyme production (3.25 U/ml) while the least enzyme was recorded on groundnut husk (2.35 U/ml) at 25. Potato peel had maximum enzyme production by Bacillus subtilis (3.05 U/ml) at pH 7.0 while the least enzyme production was from groundnut husk (2.84 U/ml) at pH 4.0.Thus there was an increase in enzyme production with corresponding increase in substrate concentration. The results obtained in this study support the suitability of using agro-industrial wastes as solid state fermentation substrates for high production of amylase. It’s also a means of solving pollution problems thus making solid state fermentation an attractive method.
Kathiresan K, Manivannan S. α Amylase production by Penicillium fellutanum isolated from mangrove rhizosphere soil. African Journal of Biotechnology. 2006;5(10):829-832.
Odee DW, Sutherland JM, Makatiani ET, Mc Inory SG, Sprent JI. Plant Soil, International training course on solid state fermentation. Document ORSTOM, Montpellier France. 2007;188:65-75.
Crueger W, Crueger A. Industrial Microbiology. Sinauer Associates, Sunderland. 2009;40:225-234.
Suganthi R, Benazir JF, Santhi R, Ramesh Kumar V, Hari A, Meenakshi N, Nidhiya KA, Kavitha G, Lakshmi R. Amylase production by Aspergillus niger under solid state fermentation using agroindustrial wastes. International Journal of Engineering Science and Technology. 2011;3(2):1756-1763.
Sodhi HK, Sharma K, Gupta JK, Soni SK. Production of a thermostable α amylase from Bacillius sp. PS-7 by solid state fermentation and its synergistic use in the hydrolysis of malt starch for alcohol production. Process Biochemistry. 2005; 40:525–534.
Pandey A, Nigam P, Soccol CR, Soccol VT, Singh D, Mohan R. Advances in microbial amylases. Biotechnology and Applied Biochemistry. 2000;31(2):135-152.
Perez-Guarre N, Torrado-Agrasar A, Lopez-Macias C, Pastrana L. Main characteristics and application of solid substrate fermentation, Electron. Journal of Environmental Agriculture and Food Chemistry. 2003;2:243–350.
Kunamneni A, Perumal K, Singh S. Amylase production in solid state fermentation by the thermophilic fungus Thermomyces Lanuginosus. Journal of Bioscience and Bioengineering. 2005;100(2):168 – 171.
Jamieson AD, Pruitt KM, Caldwell RC. An improved amylase assay. Journal of Dental Research. 2001;48(3):483-496.
Joanne MW, Lind MS, Christopher JW. Dyes and Simple Staining. The study of microbial structures. Microscopy and specimen preparation. Prescott’s Microbiology 8th Edition. Mc Graw-Hill, New-York. 2011;36-37.
Rajshree S, Rajni S. Amylase production by solid-state fermentation of agro-industrial wastes using Bacillus sp. Brazilian Journal of Microbiology. 2011;42: 1334-1342.
Ramesh MV, Lonsane BK. Effect of cultivation conditions on growth and α-amylase production by a thermophilic Bacillus sp. Biotechnology Letters. 2007;7: 501–504.
Ikram-ul-Haq H, Ashraf J, IqbalQadeer MA. Production of alpha amylase by Bacillus licheniformis using an economical medium, Bio-resources Technology. 2003;87:57- 61.
Gangadharan D, Sivaramakrishnan S, Nampoothiri KM, Pandey A. Solid Culturing of Bacillus amyloliquefaciens for Alpha Amylase Production. Food Technology and Biotechnology. 2006;44:269-274.
Chandrashekhar U, Radha IK, Basappa BK. Production of –amylase Using Banana Waste by Bacillus subtilis Under Solid State Fermentation. European Journal of Experimental Biology. 2012;2(4):1044 1052.
Raju EVN, Divakar G. Production of amylase by using Pseudomonas aeruginosa isolated from garden soil. International Journal of Advances in Pharmacy, Biology and Chemistry. 2013;2(1):50–56.
Ritesh P, Arbat T, Barkha S. Production of Glucoamylase by Aspergillus oryzae Under Solid State Fermentation Using Agro Industrial Products. International Journal of Microbiological Research. 2011;2(3):204-207.
Asgher M, Asad MJ, Rahman SU, Legge RL. A thermostable α-amylase from a moderately thermophilic Bacillus subtilis strain for starch processing. Journal of Food Engineering. 2007;79(3):950-955.
Ramesh MV, Lonsane BK. Optimization, production and partial purification of extracellular –amylase from Bacillus spp. Biotechnology Letters. 2009;11:49–52.
Krishna C, Chandrasekaran M. Selection of microorganisms which produce raw-starch degrading enzymes, Applied Microbiology and Biotechnology. 2006;27: 443-446.
Mukesh KDJ, Silambarasanb T, Renugac R, Ravi KM, Karthigai DS, Dhandapani R, Kalaichelvana PT. Production of a thermostable -amylase from Bacillus sp. PS-7 by solid state fermentation and its synergistic use in the hydrolysis of malt starch for alcohol production. European Journal of Experimental Biology. 2012;2(3):590-595.
Vipul V, Mrigank SA, Abhishek RG, Monika S, Akhilesh K. Identification and Characterization of –Amylase from Yemeni Bean seeds Euro. Journal of Experimental Biology. 2011;1(3):90-96.
Khurshid S, Ali K, Ashraf H, Qadeer MA, Rajoka I. Optimization of agro-residual medium for α‐amylase production from a hyper‐producing Bacillus subtilis KCC103 in submerged fermentation. Microbiology and Biotechnology. 2001;17:35-37.
Vasantha R, Hemashenpagam N. Production and Medium Optimization of Amylase by Bacillus using Fermentation Methods. Journal of Microbiology and Biotechnology Research. 2012;2(4):481-484.
Tanyildizi MS, Özer D, Elibol M. Optimization of α-amylase production by Bacillus sp. using response surface metho-dology. Proceedings of Biochemistry. 2007;40(7):2291-2296.
Saxena R, Singh R. Amylase production by solid-state fermentation of agro-industrial wastes using Bacillus sp. Brazilian Journal of Microbiology. 2011; 42(4):1334-1342.
Shaista K, Asghar M, Rehman K, Asad MJ. Bioprocessing of Banana Peel for Alpha-amylase production by Bacillus Subtilis, International Journal of Agriculture and Biology. 2003;6:56-78.
Basabrani BG, Devi SB, Unni W, Samanta R. Immobilization of partially purified Alpha-amylase enzyme produced by a soil born Bacillus spp. Advances in Applied Science Research. 2012;3(5):2739 -2744.
Radley JA. Industrial Uses of Starch and Its Derivatives. Applied Science Publishers Ltd, London. 2006;51-85.
Kim JH, Hosobuchi M, Kishimoto M, Seki T, Yoshida T, Taguchi H, Ryu DDY. Cellulase production by a solid state culture system. Biotechnology and Bioengineering. 2005;27:1445-1450.
Sidhu GS, Sharma P, Chakrabarti T, Gupta JK. Strain improvement for the production of a thermostable α-amylase. Enzyme and Microbiology Technology. 1997;21(7):525-530.
Mukherjee AK, Adhikari H, Rai SK. Production of alkaline protease by a thermophilic Bacillus subtilis under solid-state fermentation (SSF) condition using Imperata cylindrica grass and potato peel as low-cost medium: characterization and application of enzyme in detergent formulation. Biochemical Engineering Journal, 2008;39(2):353-361.
Ellaiah PK, Adinarayana Y, Bhavani P, Padmaja, Srinivasulu B. Optimization of process parameters for glucoamylase production under solid state fermentation by a newly isolated Aspergilus species. Process Biochemistry. 2002;38:615-620.