Standardization and Stabilization of Millet Milk by Enzyme and Its Physicochemical Evaluation

Main Article Content

K. Shunmugapriya
S. Kanchana
T. Uma Maheswari
R. Saravana Kumar
C. Vanniarajan

Abstract

Millets are nutritionally rich and occupy an important place in the diet of people in many regions of the world.  Although millets are nutritionally  superior  to  cereals, their  utilization  as  a  food  is   mostly confined  to  the traditional consumers. So, the present study was undertaken to standardize millet milk from barnyard millet, little millet, kodo millet and finger millet by enzymatic extraction method. Aqueous extract of millet milk was treated with α amylase and pasteurized at 75ºC for 15 minutes. The pasteurized millet milk was evaluated for physical and nutritional parameters. Results showed that the physical properties of developed millet milk have met the requirement of plant-based milk in terms of viscosity (2.32±0.02 to 2.82±0.03). Protein content of millet milk varied from 1.38±.0.03 to 1.12±.0.02 g. Total polyphenols (205.72±0.13 mg/100 ml) and total antioxidant activity (81.64±1.77%) were high for finger millet milk and total flavonoid content was high for barnyard millet milk (96.25±1.88 mg/100 ml). Enzymatic treatment significantly reduced the anti-nutritional factor (phytic acid, tannin and trypsin inhibitor activity) content in millet milk. The enzymatically developed product had high In vitro protein (69.28±0.28 to 85.57±1.39%) and starch digestibility (69.75±0.56 to 63.36±0.12 mg maltose/g). From the results, it was concluded that the current approach provides a convenient way for the production of nutritionally sound millet milk at the household and industrial level.

Keywords:
Millet milk, enzyme, physicochemical evaluation, total polyphenols, antioxidant activity.

Article Details

How to Cite
Shunmugapriya, K., Kanchana, S., Maheswari, T. U., Kumar, R. S., & Vanniarajan, C. (2020). Standardization and Stabilization of Millet Milk by Enzyme and Its Physicochemical Evaluation. European Journal of Nutrition & Food Safety, 12(1), 30-38. https://doi.org/10.9734/ejnfs/2020/v12i130181
Section
Original Research Article

References

Valencia‐Flores DC, Hernández‐Herrero M, Guamis B, Ferragut V. Comparing the effects of ultra‐high‐pressure homogeniza-tion and conventional thermal treatments on the microbiological, physical, and chemical quality of almond beverages. Journal of Food Science. 2013; 78(2):E199-E205.

Mäkinen OE, Wanhalinna V, Zannini E, Arendt EK. Foods for special dietary needs: Non-dairy plant-based milk substitutes and fermented dairy-type products. Critical Reviews in Food Science and Nutrition. 2016;56(3):339- 349.

Sethi S, Tyagi SK, Anurag RK. Plant-based milk alternatives an emerging segment of functional beverages: A review. Journal of Food Science and Technology. 2016;53(9):3408-3423.

Amadou I, Gounga ME, Le, GW. Millets: Nutritional composition, some health benefits and processing-A review. Emirates Journal of Food and Agriculture. 2013;501-508.

Ushakumari SR, Latha S, Malleshi NG. The functional properties of popped, flaked, extruded and roller‐dried foxtail millet (Setaria italica). International Journal of Food Science & Technology. 2004; 39(9):907-915.

Ubwa ST, Abah J, Asemave K, Shambe, T. Studies on the gelatinization temperature of some cereal starches. International Journal of Chemistry. 2012; 4(6):22.

Deora N, Deswal A. Non-dairy based beverages: An insight. Journal of Nutrition, Food Research and Technology. 2018; 1(1):1-4.

Tester RF, Qi X, Karkalas J. Hydrolysis of native starches with amylases. Animal Feed Science and Technology. 2006; 130(1-2):39-54.

Moore GRP, Canto LRD, Amante ER, Soldi V. Cassava and corn starch in maltodextrin production. Química Nova. 2005;28(4):596-600.

Jeske S, Zannini E, Arendt EK. Evaluation of physicochemical and glycaemic properties of commercial plant-based milk substitutes. Plant Foods for Human Nutrition. 2017;72(1):26-33.

AOAC. Official methods of analysis of AOAC International, (17th ed.), Gaithersburg, MD, USA: AOAC; 2000.

Ranganna S. Handbook of analysis and quality control for fruit and vegetable products. Tata McGraw-Hill Education; 1986.

Marinova D, Ribarova F, Atanassova M. Total phenolics and total flavonoids in Bulgarian fruits and vegetables. Journal of the University of Chemical Technology and Metallurgy. 2005; 40(3):255-260.

Sanjukta S, Rai AK, Muhammed A, Jeyaram K, Talukdar NC. Enhancement of antioxidant properties of two soybean varieties of Sikkim Himalayan region by proteolytic Bacillus subtilis fermenta-tion. Journal of Functional Foods. 2015;14:650-658.

Sadasivam S, Manickam A. Biochemical Methods, Ed. New Age lnternational Publishers, New Delhi. 2008;184- 185.

Hsu HW, Vavak DL, Satterlee L, Miller GA. A multienzyme technique for estimating protein digestibility. Journal of Food Science. 1977;42(5):1269-1273.

Singh U, Kherdekar MS, Jambunathan R. Studies on desi and kabuli chickpea (Cicer arietinum L.) cultivars. The levels of amylase inhibitors, levels of oligosaccharides and In vitro starch digestibility. Journal of Food Science. 1982;47(2):510-512.

Abou-Dobara MI, Ismail MM, Refaat NM. Chemical composition, sensory evaluation and starter activity in cow, soy, peanut and rice milk. Journal of Nutritional Health & Food Engineering. 2016;5(3):1-8.

Jiang S, Cai W, Xu B. Food quality improvement of soy milk made from short-time germinated soybeans. Foods. 2013; 2(2):198-212.

Cadwallader K. Instrumental measurement of milk flavour and colour. In Improving the Safety and Quality of Milk. Woodhead Publishing. 2010;181- 206.

Pineli LDLDO, Botelho RB, Zandonadi RP, Solorzano JL, De Oliveira GT, Reis CEG, Teixeira DDS. Low glycemic index and increased protein content in a novel quinoa milk. LWT-Food Science and Technology. 2015;63(2):1261-1267.

Tano-Debrah K, Asiamah K, Sakyi-Dawson E, Budu AS. Effect of malt enzyme treatment on the nutritional and physicochemical characteristics of cowpea-peanut milk. In Proceedings of the 1st International Edible Legume Conference in conjuction with the IVth World Cowpea Congress, Durban, South Africa. University of Pretoria. 2005; 1-7.

Duodu, KG Effects of processing on phenolic phytochemicals in cereals and legumes. Cereal Foods World. 2014; 59(2):64-70.

Ma Y, Huang H. Characterisation and comparison of phenols, flavonoids and isoflavones of soymilk and their correlations with antioxidant activity. International Journal of Food Science & Technology. 2014;49(10):2290-2298.

Perales-Sánchez JX, Reyes-Moreno C, Gómez-Favela MA, Milán-Carrillo J, Cuevas-Rodríguez EO, Valdez-Ortiz A, Gutiérrez-Dorado R. Increasing the antioxidant activity, total phenolic and flavonoid contents by optimizing the germination conditions of amaranth seeds. Plant Foods for Human Nutrition. 2014;69(3):196-202.

Xu B, Chang SK. Isoflavones, flavan-3-ols, phenolic acids, total phenolic profiles and antioxidant capacities of soy milk as affected by ultrahigh-temperature and traditional processing methods. Journal of Agricultural and Food Chemistry. 2009; 57(11):4706-4717.

Rodríguez-Roque MJ, Rojas-Graü MA, Elez-Martínez P, Martín-Belloso O. In vitro bioaccessibility of health-related compounds from a blended fruit juice–soymilk beverage: Influence of the food matrix. Journal of Functional Foods. 2014; 7:161-169.

Pradeep SR, Guha M. Effect of processing methods on the nutraceutical and antioxidant properties of little millet (Panicum sumatrense) extracts. Food Chemistry. 2011;126(4):1643-1647.

Hejazi SN, Orsat V, Azadi B, Kubow S. Improvement of the In vitro protein digestibility of amaranth grain through optimization of the malting process. Journal of Cereal Science. 2016; 68:59-65.

Vijayakumari K, Pugalenthi M, Vadivel V. Effect of soaking and hydrothermal processing methods on the levels of antinutrients and in vitro protein digestibility of Bauhinia purpurea L. seeds. Food Chemistry. 2007;103(3):968-975.

Joshi P, Varma K. Effect of germination and dehulling on the nutritive value of soybean. Nutrition & Food Science. 2016; 46(4):595-603.

Vagadia BH, Vanga SK, Raghavan V. Inactivation methods of soybean trypsin inhibitor–A review. Trends in Food Science & Technology. 2017;64:115- 125.

Sharma B, Gujral HS. Influence of nutritional and antinutritional components on dough rheology and In vitro protein & starch digestibility of minor millets. Food Chemistry. 2019;299:125115.

Krishnan R, Dharmaraj U, Malleshi NG. Influence of decortication, popping and malting on bioaccessibility of calcium, iron and zinc in finger millet. LWT-Food Science and Technology. 2012;48(2):169-174.

Singhal S, Baker RD, Baker SS. A comparison of the nutritional value of cow's milk and nondairy beverages. Journal of Pediatric Gastro-enterology and Nutrition. 2017;64(5):799-805.