Potentiality of Fractionated Extracts from Hemizygia welwitschii Rolfe-Ashby (Lamiaceae) Leaf to Protect Maize against Sitophilus zeamais Motschulsky (Coleoptera: Curculionidae) in Storage

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T. G. Fotso
T. Gangue
D. J. Langsi
M. N. Lakane
M. C. Zourmba
N. Tchao
W. F. G. D. Kapche
E. N. Nukenine


Laboratory tests were conducted in order to assess the potential insecticidal effect of hexane, acetone and methanol fractionated extracts from Hemizygia welwitschii leaves against Sitophilus zeamais Motschulsky, important stored maize pest. The three extracts used individually were applied at four different concentrations (2, 4, 6 and 10 g/kg of maize) against the weevil. The efficacy of the treatment was evaluated by recording adult mortality after 1, 3, 7 and 14 days of infestation, and the F1 progeny emergence. Population increase, grain damage, and seeds germination were also assessed after three months of storage. The results showed that, among the three fractionated extracts, hexane extract was the most effective with 100% mortality recorded within 14 days after infestation at the concentration of 10 g/kg of maize, followed by acetone extract with 83.75% and methanol extract with 79.21% of mortality. The LC50 values decrease with the increase of exposure periods. The 14-day LC50 values were 0.78 g/kg, 1.58 g/kg and 3.10 g/kg respectively for hexane, acetone and methanol extract. The three extracts achieved significant inhibition of F1 progeny at all the concentrations. Among them, the hexane and methanol extracts induced complete inhibition of F1 progeny emergence at 10 g/kg of maize, acetone extract recorded 82.33% of inhibition. Significant reductions of insect population growth and percentage of seed damage were recorded after three months of storage on the maize treated with each extract at all the concentrations compared to negative control. Furthermore, no alive insects was recorded in maize seeds treated with the three extracts at the concentration of 10 g/kg. It is noticed that, percentage of gain damage were similar (0.15%) in maize treated with hexane and methanol extracts at 10 g/kg, while acetone extract recorded 0.76% of grain damage. In general, these extracts had no negative effect on the germination capacity of maize grains at the end of storage. Overall, the results obtained indicate that the use of these fractionated extracts could serve as an alternative to synthetic insecticides.

Sitophilus zeamais, Hemizygia welwitschii, fractionated extracts, conservation, storage.

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Fotso, T. G., Gangue, T., Langsi, D. J., Lakane, M. N., Zourmba, M. C., Tchao, N., Kapche, W. F. G. D., & Nukenine, E. N. (2020). Potentiality of Fractionated Extracts from Hemizygia welwitschii Rolfe-Ashby (Lamiaceae) Leaf to Protect Maize against Sitophilus zeamais Motschulsky (Coleoptera: Curculionidae) in Storage. European Journal of Nutrition & Food Safety, 12(10), 68-80. https://doi.org/10.9734/ejnfs/2020/v12i1030303
Original Research Article


Ogunsina OO, Oladimeji MO, Lajide L. Insecticidal action of hexane extracts of three plants against bean weevil, Callosobruchus maculatus (F.) and maize weevil, Sitophilus zeamais Motsch. J. Ecol. Nat. Environ. 2011;3:23–28.

FAOSTAT. Food and Agricultural Organization of the United Nation; 2020. Available: http://www.fao.org/faostat/fr/#home Accessed on May 21, 2020.

Ndjouenkeu R, Fofiri NEJ, Kouebou C, Njomaha C, Grembombo AI, Mian Oudanan K. Le maïs et le niébé dans la sécurité alimentaire urbaine des savanes d’Afrique centrale. ISDA, Montpellier, France; 2010. French.

Ngamo TLS, Ngassoum MB, Mapongmetsem PM, Malaisse F, Haubruge E, Lognay G, Hance T. Current post harvest practices to avoid insect attacks on stored grains in Northern Cameroon. Agri. J. 2007;2:242–247.

Adedire CO, Obembe OM, Akinkurolere RO, Oduleye SO. Response of Callosobruchus maculatus (Coleoptera: Chrysomelidae: Bruchidae) to extracts of cashew kernels. J. Plant Dis. Prot. 2011;118:75-79.

Adedire CO. Biology, ecology and control of insect Pests of Stored Grains. In: Pest of stored cereals and pulses in Nigeria, Ofuya TI, Lale NES (Eds.). Dave Collins Publications, Nigeria. 2001;59–94.

Sagheer M, Khaliq A, Khan FZA, Gul HT, Ahmad K. Assessment of relative resistance in advanced rice genotypes in response to variation in abiotic factors and development of Tribolium castaneum (Herbst) (Coleoptera: Tenebrionidae). Inter. J. Biosci. 2013;3:33–38.

Radha R. Toxicity of three plant extracts against bean weevil, Callosobruchus maculatus (F.) and maize weevil, Sitophilus zeamais motsch. Inter. J. Cur. Res. 2014;6:6105–6109.

Nukenine EN, Monglo B, Awasom I, Tchuenguem FFN, Ngassoum MB. Farmers’ perception on some aspects of maize production and infestation levels of stored maize by Sitophilus zeamais in the Ngaoundere region of Cameroon. Cam. J. Biolo. Biochem. Sci. 2002;12:18–30.

Nwosu LC, Nwosu UI. Assessment of maize cob powder for the control of weevils in stored maize grain in Nigeria. J. Entomol. Res. 2012;36:21–24.

Starks SE, Hoppin JA, Kamel F, Lynch CF, Jones MP, Alavanja MC, Sandler DP, Gerr F. Peripheral nervous system function and organophosphate pesticide use among licensed pesticide applicators in the agricultural health study. Environ. Health Per. 2012;120:515–520.

Zhou HN., Zhao NN., Shu Shan D, Yang K, Cheng FW, Zhi LL, Yan JQ. Insecticidal activity of the essential oil of Lonicera japonica flower buds and its main constituent compounds against two grain storage insects. J. Med. Plants. Res. 2012;6(5):912–917.

Nukenine NE, Chouka PF, Vabi BM, Reichmuth C, Adler C. Comparative toxicity of four local botanical powders to Sitophilus zeamais and influence of drying regime and particle size on insecticidal efficacy. Int. J. Biol. Chem. Sci. 2013;7:1313–1325.

Ukeh DA, Birkett MA, Pickett JA, Bowman AS, Luntz AJM. Repellent activity of alligator pepper, Aframomum melegueta, and ginger, Zingiber officinale, against the maize weevil, Sitophilus zeamais. Phytochem. 2009;70:751–758.

Tandorost R, Karimpour Y. Evaluation of fumigant toxicity of original peel Citrus sinensis (L.) essential oil against three stored product insects in laboratory condition. Munis. Ent. Zool. 2012;7(1):352–358.

Kim SI, Park C, Ohh MH, Cho HC, Ahn YJ. Contact and fumigant activities of aromatic plants extracts and essential oils against Lasioderma serricorne (Coleoptera: Anobiidae). J. Stored Prod. Res. 2003;39:11–19.

Kosini D, Nukenine EN, Tofel KH. Efficacy of Cameroonian Ocimum canum Sims (Lamiaceae) leaf extract fractions against Callosobruchus maculatus (F.) (Coleoptera: Chrysomelidae), infesting Bambara groundnut. J. Entomol. Zool. Stud. 2015;3(5):487–494.

Fotso TG, Tofel HK, Abdou JP, Tchao N, Zourmba CM, Adler C, Nukenine EN. Control of Callosobruchus maculatus (Coleoptera: Chrysomelidae) using three different extracts from Cameroonian Hemizygia welwitschii (Lamiaceae) leaves powder on stored Vigna unguiculata (Fabaceae). J. Insect Sci. 2019;19(2):22,1–9.

Kosini D, Nukenine EN, Tofel KH, Goudoungou JW, Langsi DJ, Adamou M, Abdou JP, Djafsia B, Ndouwe HMT. Impact of Environment on Callosobruchus maculatus (Coleoptera: Chrysomelidae) Response to Acetone Extract of Gnidia kaussiana Meisn (Thymeleaceae) and Ocimum canum Sims (Lamiaceae) Botanical Insecticides. Euro. J. Nut. Food Saf. 2020;12(8):128–139.

Fotso TG, Nukenine EN, Tchameni R, Goudoungou JW, Kosini D, Tigamba V, Adler C. Use of Cameroonian Hemizygia welwitschii Rolfe Ashby (Lamiaceae) leaf powder against Callosobruchus maculatus and Sitophilus zeamais. J. Entomol. Zool. Stud. 2018;6(4):1261–1269.

AFNOR (Association Française de Normalisation). Recueil des normes françaises des produits dérivés des fruits et légumes : Jus de fruits. 1ère édition. Paris, France : AFNOR. 1982;327. French.

Adeniyi SA, Orjiekwe CL, Ehiagbonare JE, Arimah BD. Preliminary phytochemical analysis and insecticidal activity of ethanolic extracts of four tropical plants (Vernonia amygdalina, Sida acuta, Ocimum gratissimum and Telfaria occidentalis) against beans weevil (Acanthscelides obtectus). Int. J. Phys. Sci. 2010;5:753–762.

Nukenine EN, Adler C, Reichmuth C. Bioactivity of fenchone and Plectranthus glandulosus oil against Prostephanus truncatus and two strains of Sitophilus zeamais. J. Appl. Entomol. 2010;134:132–141.

Abbott WSA. Method of computing the effectiveness of insecticide. J. Econ. Entomol. 1925;18:265–267.

Rao NK, Hanson J, Dulloo ME, Ghosh K, Nowell D, Larinde M. Manuel de manipulation des semences dans les banques de gènes. Manuels pour les banques de gènes No. 8. Biodiversité Internationale, Rome, Italie. 2006;165. French.

Demissie G, Tefera T, Tadesse A. Efficacy of SilicoSec, filter cake and wood ash against the maize weevil, Sitophilus zeamais Motschulsky (Coleoptera: Curculionidae) on three maize genotypes. J. Stored Prod. Res. 2008;44:227– 231.

Finney DJ. Probit analysis. Cambridge University. Press. London; 1971.

Sarwar M. The Killer Chemicals for Control of Agriculture Insect Pests: The Botanical Insecticides. Inter. J. Chem. Biomol. Sci. 2015;1(3):123–128.

Moreira MD, Picanço MC, Barbosa LCdeA, Guedes RNC, de Campos MR, Silva GA, Martins JC. Plant compounds insecticide activity against Coleoptera pests of stored products. Pesq. agropec. bras. Brasília. 2007;42(7):909–915.

Polosky J, Varon Z, Arnoux B, Pascard C, Petit GR, Schmidt JM, Ochi M, Kotsuki H. Cytotoxic effect of terpenoids from Aphanomixis grandiflora. Phytochem. 1979;30:1198–1201.

Kostyukovsky M, Rafaeli A, Gileadi C, Demchenko N, Shaaya E. Activation of octopaminergic receptors by essential oil constituents isolated from aromatic plants: possible mode of action against insect pests. Pest Manag. Sci. 2002;58:1101–1106.

Rubabura K, Nsambu M, Muhigwa B, Bagalwa M, Bashwira S. Evaluation in vitro activity of insect alkaloid, saponins, terpenoids or steroids extracts capscicum frutescens L. (Solanaceae) against Antestiopsis orbitalis ghesquierei, pests of coffee trees. Inter. J. Inno. Appl. Stud. 2014;8(3):1231–1243.

Aniszewski T. Alkaloids-secrets of life: alkaloid chemistry, biological significance, applications and ecological role. Elsevier, Amsterdam. 2007;185-186.

Faraway JJ, Practical Regression and Anova using R; 2002. Published on the URL: http://wwwcranr-projectorg/doc/contrib/ Faraway-PRApdf Accessed 3rd February 2010.

Khoshnoud H, Ghiyasi M, Amimia R, Fard SS, Tajbakhsh M, Salehzadeh H, Alahyary P. The potentials of using inject properties of medicinal plants against insect pests. Pak. J. Biol. Sci. 2008;11:1–5.

Akinbuluma MD, Adepetun MT, Yeye EO. Insecticidal Effects of Ethanol Extracts of Capsicum Frutescens and Dennettia Tripetala against Sitophilus Zeamais Motschulsky on Stored Maize. Inter. J. Res. Agri. For. 2015;2(11):1–7.

Ileke KD, Ogungbite OC. Entomocidal activity of powders and extracts of four medicinal plants against Sitophilus oryzae (L), Oryzaephilus mercator (Faur) and Ryzopertha dominica (Fabr.). Biol. Sci. 2014;7:57–62.

Chebet F, Deng AL, Ogendo JO, Kamau AW, Bett PK. Bioactivity of Selected Plant Powders against Prostephanus truncatus. Plant Prot. Sci. 2013;49:34–43.

Adesina JM, Jose AR, Rajashaker Y, Afolabi LA. Entomo Toxicity of Xylopia aethiopica and Aframomum melegueta in suppressing oviposition and adult emergence of Callasobruchus maculatus (Fabricus) (Coleoptera: Chrysomelidae) infesting stored cowpea seeds. Jordan J. Biol. Sci. 2015;8:263–268.

Adel MM, Sehnal F, Jurzysta M. Effects of alfalfa saponins on the moth Spodoptera littoralis. J. Chem. Ecol. 2000;26:1065–1078.

Rahuman AA, Gopalakrishnan G, Venkatesan P, Geetha K. Isolation and identification of mosquito larvicidal compound from Abutilon indicum (Linn.) Sweet. Parasitol. Res. 2008;102:981–988.

Silva-Aguayo G. Botanical Insecticides. Radcliffe’s IPM World Text Book. Regents of the University of Minnesota; 2013.

Taylor WG, Fields PG, Sutherland DH. Insecticidal components from field pea extracts: Soyasaponins and lysolecithins. J. Agri. Food Chem. 2004;52:7484–7490.

Balandrin MF. Commercial utilization of plant-derived saponins: An overview of medicinal, pharmaceutical, and industrial applications. In G. R. Waller, K. Yamasaki (Eds.), Saponins used in traditional medicine. New York: Plenum Press; 1996;1–14.

Applebaum SW, Marco S, Birk Y. Saponins as possible factor of resistance of legume seeds to the attack of insects. J. Agri. Food Chem. 1969;17:618–622.

Stevenson PC, Dayarathna TK, Belmain SR, Veitch NC. Bisdesmosidic saponins from Securidaca longepedunculata roots: Evaluation of deterrency and toxicity to Coleopteran storage pests. J. Agri. Food Chem. 2009;57:8860–8867.

Pingsutiwong W, Wattanakij P. Statistic Export-Import Seed Control for Commercial. Plant. Agri. Equip. Control Div. Bangkok. 1996;51.