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In the current study, calcium lactate (ca-lactate) was used as a calcium source and tripotassium citrate (TPC) as chelating agents to produce calcium-enriched soymilk with calcium content equivalent to cow’s milk (120 mg/100mL). Physicochemical properties of calcium-enriched soymilk, including nutritional composition, pH, titratable acidity, particle diameter, sedimentation, viscosity, ion conductivity, and sensory evaluation were investigated. Our results showed significant differences (P<.05) in moisture, ash content, titratable acidity, and ion conductivity after calcium and TPC were added. Moreover, the addition of calcium decreased the pH of soymilk from 6.69 to 6.21-6.51. The higher concentration of calcium also increased the calcium content, particle diameter, sedimentation, and viscosity, while the reverse results were shown when TPC was added. The intensities perceived of mouthfeel, visual appearance, and overall acceptability were greatly varied among calcium-enriched soymilks. The exact proportion of ca-lactate and TPC were able to produce calcium-enriched soymilk regarding higher stability during storage and great acceptability of the final product.
Bricarello LP et al. Comparison between the effects of soy milk and non-fat cow milk on lipid profile and lipid peroxidation in patients with primary hypercholesterolemia. Nutrition; 2004. DOI: 10.1016/j.nut.2003.10.005
Gerstner G. How to fortify beverages with calcium. Food Mark. Technol. 2003;16–19.
USDA, National Nutrient Database for Standard Reference, Release28. U.S. Department of agriculture, agricultural research service; 2015.
Prabharaksa C, Olek AC, Steinkraus KH. Enrichment of soybean milk with calcium. acta biotechnol. 1989;9(1):9–16. DOI: 10.1002/abio.370090103
Trailokya A, Srivastava A, Bhole M, Zalte N. Calcium and calcium salts. J. Assoc. Physicians. India. 2017;65:100–103.
Pathomrungsiyounggul P, Grandison AS, Lewis MJ. Effect of calcium carbonate, calcium citrate, tricalcium phosphate, calcium gluconate and calcium lactate on some physicochemical properties of soymilk. Int. J. Food Sci. Technol. 2010;45(11):2234–2240. DOI: 10.1111/j.1365-2621.2010.02399.x
Pathomrungsiyounggul P, Lewis MJ, Grandison AS. Calcium-fortified soymilk in handbook of food fortification and health: From concepts to public health applications; 2013.
Qiuyan J. Xianzhen K Yufei H. The effects of oxygen-insulated grinding process on the quality of yuba. Food and Machinery. 2019;35(6):177–181. DOI:10.13652/j.issn.1003-5788.2019.06.32 AOAC. Official Methods of Analysis of AOAC International; 2005.
Zhao L. Chen Y, Yan Z, Kong X, Hua Y. Physicochemical and rheological properties and oxidative stability of oil bodies recovered from soybean aqueous extract at different pHs. Food Hydrocoll; 2016. DOI: 10.1016/j.foodhyd.2016.06.032
Wangcharoen W. Nutrition data and antioxidant capacity of soy milk ice cream and black sesame flavoured soy milk ice cream. Asian J. Food Agro-Industry; 2008.
Pathomrungsiyounggul P, Grandison AS, Lewis MJ. Effects of calcium chloride and sodium hexametaphosphate on certain chemical and physical properties of soymilk. J. Food Sci. 2007;72(8). DOI: 10.1111/j.1750-3841.2007.00504.x
Navicha WB, Hua Y, Masamba K, Kong X, Zhang C. Distribution of odour compounds, antinutritional factors and selected storage stability parameters in soymilk as affected by differences in roasting temperatures and times. J. Food Meas. Charact. 2018; 12(3):1695–1706. DOI: 10.1007/s11694-018-9785-5
Tyl C, Sadler GD. pH and Titratable Acidity in Food Analysis Fifth Edition; 2017.
ISO-8586:2012. Sensory analysis — General guidelines for the selection, training and monitoring of selected assessors and expert sensory assessors. Int. Organ. Stand.; 2012.
Lawless HT, Heymann H. Sensory evaluation of food: Principles and practices. 2010; 2nd Ed.
Yazici F, Alvarez VB, Mangino M E, Hansen PMT. Formulation and processing of a heat stable calcium-fortified soy milk. Journal of Food Science. 1997;62(3):535–538. DOI: 10.1111/j.1365-2621.1997.tb04424.x
Irshad A, Sharma BD, Ahmed SR, Talukder S, Malav OP, Kumar A. Effect of incorporation of calcium lactate on physico-chemical, textural, and sensory properties of restructured buffalo meat loaves. Vet. World; 2016. DOI: 10.14202/vetworld.2016.151-159
Bellows L, Moore R. Potassium and the Diet. Food and Nutrition Series|Health. 2013;9.355(9):1.
Pappas CP, Rothwell J. The effects of heating, alone or in the presence of calcium or lactose, on calcium binding to milk proteins. Food Chem.; 1991. DOI: 10.1016/0308-8146(91)90033-K
Horne DS, Dalgleish DG. Electrostatic interaction and the kinetics of protein aggregation: αs1-casein. Int. J. Biol. Macromol.; 1980. DOI: 10.1016/0141-8130(80)90067-7
Ono T. SOY (SOYA) CHEESES. In Encyclopedia of Food Sciences and Nutrition; 2003.
Ye A, Singh H. Influence of calcium chloride addition on the properties of emulsions stabilized by whey protein concentrate. Food Hydrocoll. 2000;14(4): 337–346. DOI: 10.1016/S0268-005X(00)00010-2
Keowmaneechai E, McClements DJ. Influence of EDTA and citrate on physicochemical properties of whey protein-stabilized oil-in-water emulsions containing CaCL2. J. Agric. Food Chem., 2002;50(24):7145–7153. DOI: 10.1021/jf020489a
Pathomrungsiyounggul P, Lewis MJ, Grandison AS. Effects of calcium-chelating agents and pasteurisation on certain properties of calcium-fortified soy milk. Food Chem. 2010;118(3)808–814. DOI: 10.1016/j.foodchem.2009.05.067
Saeidy S, Keramat J, Nasirpour A. Physicochemical properties of calcium-fortified soymilk with microencapsulated and chelated calcium salt. European Food Research and Technology. 2014;238(1):105–112. DOI: 10.1007/s00217-013-2090-y
Molina MI. Wagner JR. The effects of divalent cations in the presence of phosphate, citrate and chloride on the aggregation of soy protein isolate. Food Res. Int.; 1999. DOI: 10.1016/S0963-9969(99)00086-1
Pare A, Yadav BK, Santhakumaran A. Estimation of Electrical Conductivity of Soymilk with Varied TSS Levels using Ohmic Heating; 2015.
Rasyid F, Hansen PMT. Stabilization of soy milk fortified with calcium gluconate. Top. Catal. 1991;4(5):415–422. DOI: 10.1016/S0268-005X(09)80136-7
Jensen AS, Unangst MR. Conductivity of calcium salts. J. Chem. Phys. 1941;9(2):195. DOI: 10.1063/1.1750878
Kinsella JE. Functional properties of soy proteins. J. Am. Oil Chem. Soc.; 1979. DOI: 10.1007/BF02671468
Kroll RD. Effect of pH on the binding of Calcium Ions by Soybean Proteins. Cereal Chem. 1984;61:490–495.
Ono T, Katho S, Mothizuki K. Influences of Calcium and pH on Protein Solubility in Soybean Milk. Biosci. Biotechnol. Biochem. 1993;57(1):24–28. DOI: 10.1271/bbb.57.24
Lu JY, Carter E, Chung R. A. Use of Calcium Salts for Soybean Curd Preparation. J. Food Sci. 1980;45(1):32–34. DOI: 10.1111/j.1365-2621.1980.tb03864.x
Mgaya-Kilima B, Remberg SF, Chove BE, Wicklund T. Influence of storage temperature and time on the physicochemical and bioactive properties of roselle-fruit juice blends in plastic bottle. Food Science & Nutrition. 2014;2(2):181–191. DOI: 10.1002/fsn3.97
Hinds MJ, Chinnan MS, Beuchat LR. Particle size distribution in a heat-processed beverage prepared from roasted peanuts. Food Res. Int. 1997; 30(1):59–64. DOI: 10.1016/S0963-9969(97)00016-1
Daengprok W, Garnjanagoonchorn W, and Mine Y. Fermented pork sausage fortified with commercial or hen eggshell calcium lactate. Meat Sci.; 2002. DOI: 10.1016/S0309-1740(01)00247-9
Gupta SK, Karahadian C, Lindsay RC. Effect of Emulsifier Salts on Textural and Flavor Properties of Processed Cheeses. J. Dairy Sci.; 1984 DOI: 10.3168/jds.S0022-0302(84)81367-3