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The study aimed at evaluating the amino acid profile, fatty acid profile, anti-nutritional factors, functional properties and in-vitro antioxidant activities of Cucurbita maxima and Cucurbita mixta fruit pulps and seeds. Freshly harvested Cucurbita mixta and Cucurbita maxima fruit were processed into flour as; Pa: Cucurbita maxima pulp flour, Pi: Cucurbita mixta pulp flour, Sa: Cucurbita maxima seed flour and Si: Cucurbita mixta seed flour and were evaluated for amino acid profile, fatty acid profile, anti-nutritional factors, functional properties and in-vitro antioxidant activities. Triplicate data were analysed and means were separated using New Duncan Multiple Range Test (NDMRT) at p<0.05. The protein content of the fruit pulps and seeds flour samples were 12.77 g/100 g (Pi), 13.22 g/100 g (Pa), 15.37 g/100 g (Sa) and 16.86 g/100 g (Si). Total essential amino acid was 5.33 mg/100 g of protein (Pa) 6.62 mg/100 g of protein (Pi), 9.85 mg/100 g of protein (Sa) and 14.61 mg/100 g of protein (Si). Total essential amino acid of Pi and Si are significantly higher (p>0.05) than Pa and Sa. Statistically, saturated fatty acid and polyunsaturated fatty acid of the Pi and Si were significantly lower (p<0.05) than Pa and Sa respectively. Antioxidant activities against ABTS* (Pi) is significantly higher (p>0.05) than Pa and with no significant differences (p<0.05) between ABTS values of Si and Sa. While there is a significant difference (p<0.05) between Si and Sa as well as Pi and Pa antioxidant activities against DPPH* respectively. In conclusion, Cucurbita seeds and pulps flour contains high protein content, appreciable amount of essential minerals, lower Na/K molar ratio of less than one and they also exhibit a good free radical scavenging abilities against DPPH*, ABTS* and ability to reduce Fe3+ to Fe2+ with high content of total phenol and flavonoid.
Maggs GK, Madesen S, Chritiansen JL. Genetic marker techniques in the family Cucurbitaceae. Genetic Resources and Crop Evolution. 2000;47:385-393.
Zhang Y, Zhou J, Wu T, Cao J. Shoot regeneration and the relationship between organogenic capacity and endogenous hormonal contents in pumpkin. Plant Cell, Tissue and Organ Culture. 2008;93:323–33.
FAOSTAT. Food Agricultural Organisation Statistics on World agricultural data; 2007.
Obembe OO, Aworunse OS, Bello OA, Ani AO. Multiple shoots induction from indigenous Nigerian Pumpkin (Cucurbita pepo L.). Annual Research and Review in Biology, ARRB. 35756; 2017. ISSN: 2347-565X.
Pal SP, Alam I, Anisuzzaman M, Sarker KK. Indirect organogenesis in Summer Squash (Cucurbita pepo L.). Turkey Journal of Agriculture and Forestry. 2007;31:63-70.
Akinsanmi O. Certificate Agricultural Science. Ile-Ife, Nigeria. Longman Group Limited, London. 1980;196.
Ogbu IM, Ajiwe VIE. Biodiesel production via esterification of free fatty acids from Cucurbita pepo L. seed oil: Kinetic studies. International Journal of Science and Technology. 2013;2:616-620.
Duke JA, Ayensu ES. Medicinal plants of China. Reference Publications, Inc, Algonac. 1985;2.
Oloyede FM. Growth, yield and antioxidant profile of pumpkin (Cucurbita pepo L.) leafy vegetable as affected by NPK compound fertilizer. Journal of Soil Science and Plant Nutrition. 2012;12(3):379-388.
Veljkovic S. Nutritional and dietetic value of Pumpkin (C. pepo, L.). Hrana-I-Ishrana. 1992;33:137-139.
AOAC. Association of official analytical chemist. Official Methods of Analysis of the Analytical Chemist International, 18th Ed. Gathersburg, MD USA; 2012.
Spackman DH, Stein WH, Moore S. Chromatography of amino acids on sulphonated polystyrene resins: An improved system analytical chemistry. 1958;30:1190-1205.
Pintér-Szakács M, Molnán-Perl I. Determination of tryptophan in unhydrolyzed food and feedstuffs by the acid Ninhydrin method. Journal of Agriculture and Food Chemistry. 1990;38: 720-726.
Markwell AK, Mary M, Haas S, Bieber L, Tolbert NE. A modification of the Lowry procedure to simplify protein determination in membrane and lipoprotein samples. Analytical Biochemistry. 1978;87:206-210.
Solomon M, Owolawashe H. The analyses of amino acid, fatty acid and mineral in a legume-cereal based complementary food blend used in Jos, Nigeria. The Internet Journal of Nutrition and Wellness. 2006;4(1):1-8.
Medoua GN, Mbome IL, Agbor-Egbe T, Mbofung. Influence of fermentation on some quality characteristics of trifoliate yam (Dioscorea dumetorum) hardened tubers. Food Chemistry. 2007;107(3): 1180-1186.
Ukpabi A, Ejidoh EO. Experimental procedures for food and water analysis. San Press Publishers, Enugu, Nigeria. 1989;89.
Abulude FO. Effect of processing on nutritional composition, phytate and functional properties of rice (Oryza sativa L) flour. Nigerian Food Journal. 2004;22: 97-104.
Obadoni BO, Ochuko PO. Phytochemical studies and comparative efficacy of the crude extracts of some homeostatic plants in Edo and Delta States of Nigeria. Global Journal of Pure and Applied Science. 2001;8:203-208.
Adebowale YA, Adeyemi IA, Oshodi AA. Functional and physico-chemical properties of flour of six mucuna species. African Journal of Biotechnology. 2005a;4(12):1461-1468.
Sathe S, Salunkhe K. Functional properties of the great Northern Bean (Phaseolus vulgaris L.) proteins: Emulsion, foaming, viscosity, and gelation properties. Journal of Food Science. 2006;46:71-81.
Deshpande S, Sathe SP, Cornforth D, Salunkhe K. Effects of dehulling on functional properties of dry bean (Phaseolus vulgaris L.) flours. NFS Faculty Publications. 1982;59.
Adebowale KO, Olu-Owolabi BI, Olawumi EK, Lawal OS. Functional properties of native, physically and chemically modified breadfruit (Artocarpus artillis) starch. Industrial Crops Production. 2005b;21:343-351.
Asoegwu SN, Ohanyere SO, Kanu OP, Iwueke CN. Physical properties of African oil bean seed (Pentonclethra nacrophylla). Agricultural Engineering International Journal. 2006;44:6-12.
Onweluzo JC, Nwabugwu CC. Fermentation of millet (Pennisetum americanum) and pigeon pea (Cajanus cajun) seeds for flour production: Effects on composition and selected functional properties. Pakistan Journal of Nutrition. 2009;8:737-744.
Aluko RE, Monu E. Functional and bioactive properties of quinoa seed protein hydrolysates. Journal of Food Science. 2003;68:1254–1258.
Re R, Pellegrin N, Proteggente A, Pannala A, Yang M, Rice-Evans C. Antioxidant activity applying an improve ABTS radication decolourization assay. Free Radical Biology and Medicine. 1999;26: 1231-1237.
Singleton VL, Orthofer R, Lamuela-Raventos RM. Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin-Ciocalteu reagent. Methods Enzymology. 1999;299: 152-177.
Bushra S, Farooq A, Muhammad A. Effect of extraction solvent/technique on the antioxidant activity of selected medicinal plant extracts. Molecules. 2009;14:2167-2180.
FAO. Food and Agriculture Organisation of the United Nations. Panicum maximum, guinea grass, colonial grass, Tanganyika grass; 2003.
Kim MY, Kim EJ, Kim YN, Choi C, Lee BH. Comparison of the chemical compositions and nutritive values of various pumpkin (Cucurbitaceae) species and parts. Nutrition Research and Practice. 2012;6: 21–27.
Achilonu MC, Nwafor IC, Umesiobi DO, Sedibe MM. Biochemistry proximate of pumpkin (Cucurbitaeae spp) and their beneficial effects on the general well-being of poultry. Journal of Animal Physiological and Animal Nutrition. 2017;1-13.
Karanja JK, Mugendi JB, Fathiya MK, Muchugi AN. Comparative study on the nutritional value of the pumpkin, Cucurbita maxima varieties from different regions in Kenya. Department of Biochemistry and Biotechnology, Kenyatta University, Nairobi, Kenya. 2005;1-12.
Aremu MO, Olaofe O, Akintayo ETA. Comparative study on the chemical and amino acid composition of some Nigerian underutilized legume flours. Pakistan Journal Nutrition. 2006;5:34-38.
Anuonye JC, Jigam AA, Ndaceko GM. Effects of extrusion-cooking on the nutrient and anti-nutrient composition of pigeon pea and unripe plantain blends. Journal of Applied Pharmaceutical Science. 2012;2(5):158-162.
Alfawaz A. Chemical composition and oil characteristics of pumpkin (Cucurbita maxima) seed kernels. Resources Buling, (129), Food Science and Agriculture Resources Center, King Saud University. 2004;5-18.
Guillon F, Champ M. Structural and physical properties of dietary fibres, and consequences of processing on human physiology. Food Research International. 2000;32:233–245.
American Diabetes Association. Standards of medical care in diabetes 2013. Diabetes Care. 2013;36(1):S11–S66.
Oyarekua MA. The effects of ungerminated cowpea on the nutritional quality of germinated maize. International Journal of Biotechnology and Food Science. 2010;2(2):35-43.
Ishida H, Suzuno H, Sugiyama N, Innami S, Todokoro T, Maekawa A. Nutritional evaluation of chemical component of leaves stalks and stems of sweet potatoes (Ipomea batatas poir). Food Chemistry. 2000;68:359-367.
Fawzi WW, Hunter DJ. Vitamins in HIV disease progression and vertical transmission. Epidemiology. 1998;9:457-466.
FND. Food and nutrition board dietary reference intake for energy, carbohydrate, fibre, fat, fatty acids, cholesterol, protein and amino acid (micro-nutrient Institute of medicine. National Academy of Sciences. The National Academies Press Washington D. C. 2002;107-967.
Gibson RS. Inadequate intakes of zinc in developing countries-Practical household strategies to reduce risk of deficiency. Zinc and Human Health; 2003.
(Accessed on 15th May 2007)
FAO/WHO/UNU. Protein and amino acid requirements in human nutrition. WHO Press. 2007;150.
Badamosi EJ, Ibrahim LM, Temple VJ. Nutritional evaluation of a locally formulated weaning food. JUTH_PAP. West African Journal of Biology. Science. 1995;3:85-93.
Temple VJ, Badamosi EJ, Ladeji O, Solomon M. Proximate chemical composition of three locally formulated complementary foods. West African Journal of Biological Science. 1996;5:134-143.
Fernandez DR, Vanderjagt DJ, Williams M, Huang YS, Chuang LT. Fatty acids, amino acids and trace mineral analyses of five weaning foods from Jos, Nigeria. Plants Foods Human Nutrition. 2002;57:257- 274.
Ojiako IA, Asumugha GN, Ezedinma CNE. Analysis of production trends in the major root and tuber crops in Nigeria, 1961-2005. Research in Crops. 2007;8(2):371-380.
Oluwajuyitan TD, Ijarotimi OS. Nutritional, antioxidant, glycaemic index and antihyperglycaemic properties of improved traditional plantain-based (Musa ABB), dough meal enriched with tigernut (Cyperus esculentus) and defatted soybeans (Glycine max) cake for diabetics patients. Heliyon Elsevier. 2019;e1504-9.
Smith DL. Calcium oxalate and carbonate deposits in plant cells. In: The Role of Calcium in Biological Systems, Anghileri, L.J. and A.M. Tuffet-Anghileri (Eds.). CRC Press, Florida, USA. 1982;253-226.
Ogunkoya MO, Abulude FO, Oni AB. Determination of anatomical, proximate, minerals, oxalate, tannin and phytate compositions of Cuban Boa (Epicrates anquifer). Electron. Journal of Environmental. Agricultural and Food Chemistry. 2006;5:1160-1166.
Ogunlade I, Ilugbiyin A, Osasona AI. A comparative study of proximate composition, antinutrient composition and functional properties of Pachira glabra and Afzelia africana seed flours. African Journal of Food Science. 2011;5(1):32–35.
Nnam NM. Chemical evaluation of mulitimixes formulated from some local staples for use as complementary foods in Nigeria. Plant Food and Human Nutrition. 2000;55:255-263.
Akubor PI. Evaluation of physiochemical and sensory properties of soybean-sweet potato supplementary food. Journal of Chemical Society of Nigeria. 2008;33:112-121.
Karunna D, Noel G, Dilip K. Production and use of raw potato flour in Maruritanian traditional foods. Food and Commercialition Bulletin, United National University. 1996;17(2).
Thierry NN, Mbougueng P, Léopold TN, Mbofung C. Functional properties of Cucurbita maxima Duchense, Cucumeropsis mannii Naudin and Lagenaria sciceraria (Molina) Standley defatted seed flours. Journal of Food Measurement and Characterization; 2017.
Kulkarni KD, Kulkarni DN, Ingle UM. Sorghum Malted and soya bean weaning food formulations: Preparation, functional properties and nutritive value. Food Nutrition Bulletin. 1991;13:322-327.
Cameron R, Hofvander S. Target food sources for formulating complementary feeding and given improper diets; UNNESCO/NES. 1983;16.
Kempka AP, Honaiser TC, Fagundes E, Prestes RC. Functional properties of soy protein isolate of crude and enzymatically hydrolysed at different times. International Food Resources Journal. 2014;22(6): 2229–2236.
Marianna N, Xanthopoulou T, Elizabeth F, Smaragdi A. Antioxidant and lipoxygenase inhibitory activities of pumpkin seed extracts. Food Research International. 2009;1:300-312.
Ragaee S, Abdel-Aal EM. Pasting properties of starch and protein in selected cereals and quality of their food products. Food Chemistry. 2006;95:9-18.
FAO/WHO. CODEX CAC/GL 08, 1991. Codex Alimentarius: Guidelines on Formulated Supplementary Foods for Older Infants and Young Children. (4). FAO/WHO Joint Publications. 1991;144.
Morris ER, Ellis R. Usefulness of the dietary phytic acid/zinc molar ratio as an index of zinc bioavailability to rats and humans. Biological Trace Element Research. 1985;19:107-117.
Hurrell RF. Influence of vegetable protein sources on trace elements and minerals bioavailability. Journal of Nutrition. 2003;133:2973S-2977S.
Aina JO, Oyeleke WA. Production of jam and sauce from African Pumpkin (Cucurbita mixta) Pulp. Iree Journal of Science and Engineering. 2005;5(1):12- 36.