Characterization of Anthocyanins in Sweet Potato Leaves Grown in Various Stages and Conditions

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Xiaoyu Su
Zhenbao Jia
Fei Tao
Jiamin Shen
Jingwen Xu
Jason Griffin
Weiqun Wang


Phytochemical-enriched edible greens, sweet potato leaves (Ipomoea batatas L.), have become popular due to potential health benefits. However, the phytochemical contents in sweet potato leaves and their subsequent change over harvest stages and growth condition are mostly unknown. In this study, the anthocyanin profile and content in leaves of four sweet potato cultivars, i.e., white-skinned and white-fleshed Bonita, red-skinned and orange-fleshed Beauregard, red-skinned and white-fleshed Murasaki and purple-skinned and purple-fleshed P40, were evaluated. Fourteen anthocyanins were isolated and identified by HPLC-MSI/MS. The most abundant was cyanidin 3-caffeoyl-p-hydroxybenzoyl sophoroside-5-glucoside, which comprised up to 20% of the total anthocyanins. Of the young leaves (1st and 2nd slip cuttings), Bonita contained the highest anthocyanin content followed by P40. Of the mature leaves (vine stage), Beauregard had the greatest anthocyanin (592.5 ± 86.4 mg/kg DW) and total phenolic (52.2 ± 3 mg GAE/g DW). It should be noted that the lowest anthocyanin and total phenolic content of shoots were found in P40, while tubers of P40 contain the highest content of each. Furthermore, the increase in leaf anthocyanin content over the growth stages that was observed in three of the cultivars but not in P40. No significant difference of anthocyanin content was found in Beauregard leaves grown in the high tunnels when compared with that in the open field. This study demonstrated for the first time that anthocyanin levels were significantly changed in response to various growth stages but not high tunnel condition, indicating that the effect of anthocyanin biosynthesis in sweet potato leaves is highly variable and genotype specific.

Anthocyanins, growth stages, HPLC-ESI/MS, sweet potato leaves.

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How to Cite
Su, X., Jia, Z., Tao, F., Shen, J., Xu, J., Griffin, J., & Wang, W. (2019). Characterization of Anthocyanins in Sweet Potato Leaves Grown in Various Stages and Conditions. European Journal of Nutrition & Food Safety, 10(4), 253-262.
Original Research Article


Bovell-Benjamin AC. Sweet potato: A review of its past, present and future role in human nutrition. Advances in Food and Nutrition Research. 2007;1–59.

Claessens L, Stoorvogel JJ, Antle JM. Ex ante assessment of dual-purpose sweet potato in the crop-livestock system of western Kenya: A minimum-data approach. Agric. Syst. 2008;99(1):13–22.

Islam S. Sweet potato (Ipomoea batatas L.) leaf: Its potential effect on human health and nutrition. J. Food Sci. 2006;71 (2):13–21.

Islam MS, Yoshimoto M, Yahara S, Okuno S, Ishiguro K, Yamakawa O. Identification and characterization of foliar polyphenolic composition in sweet potato (Ipomoea batatas L.) genotypes. J. Agric. Food Chem. 2002;50(13):3718–3722.

Yoshimoto M, Okuno S, Islam MS, Kurata RA, Yamakawa O. Polyphenolic content and antimutagenicity of sweet potato leaves in relation to commercial vegetables. In Acta Horticulturae. 2003; 628:677–685.

Parker P, Kram KE, Hall DTT. Peer coaching: An untapped resource for development. Organ. Dyn. 2014;43(2): 122–129.

Islam MS, Yoshimoto M, Terahara N, Yamakawa O. Anthocyanin compositions in sweet potato (Ipomoea batatas L.) leaves. Biosci. Biotechnol. Biochem. 2002; 66(11):2483–2486.

Su X, Griffin J, Xu J, Ouyang P, Zhao Z, Wang W. Identification and quantification of anthocyanins in purple-fleshed sweet potato leaves. Heliyon. 2019;5(6): e01964.

Islam M, Yoshimoto M, Yamakawa O. Distribution and physiological functions of caffeoylquinic acid derivatives in leaves of sweet potato genotypes. J. Food Sci. 2003;68(1):111–116.

Meyer AS, Heinonen M, Frankel EN. Antioxidant interactions of catechin, cyanidin, caffeic acid, quercetin and ellagic acid on human LDL oxidation. Food Chem. 1998;61(1–2):71–75.

Lim S, Xu J, Kim J, Chen TY, Su X, Standard J, Carey E, Griffin J, Herndon B, Katz B, et al. Role of anthocyanin-enriched purple-fleshed sweet potato p40 in colorectal cancer prevention. Mol. Nutr. Food Res. 2013;57(11):1908–1917.

Johnson M, Pace RD. Sweet potato leaves: Properties and synergistic interactions that promote health and prevent disease. Nutr. Rev. 2010;68(10): 604–615.

Xu J, Su X, Li Y, Sun X, Wang D, Wang, W. Response of bioactive phytochemicals in vegetables and fruits to environmental factors. Eur. J. Nutr. Food Safety. 2019;9 (3):233-247.

Shao HB, Chu LY, Lu ZH, Kang CM. Primary antioxidant free radical scavenging and redox signaling pathways in higher plant cells. Int. J. Biol. Sci. 2008;4 (1):8–14.

Shao H bo, Chu L ye, Shao M an, Ja hleel CA, Hong-mei M. Higher plant antioxidants and redox signaling under environmental stresses. Comptes Rendus - Biologies. 2008;433–441.

Çirak C, Radušiene J, Çamas N. Pseudohypericin and hyperforin in two Turkish Hypericum species: Variation among plant parts and phenological stages. Biochem. Syst. Ecol. 2008;36(5-6): 377–382.

Omezzine F, Haouala R. Effect of Trigonella foenum-graecum L. development stages on some phytochemicals content and allelopathic potential. Sci. Hortic. (Amsterdam). 2013; 160:335–344.

Taylor JLS, Van Staden J. The effect of age, season and growth conditions on anti-inflammatory activity in Eucomis autumnalis (Mill.) Chitt. Plant extracts. Plant Growth Regul. 2001;34(1): 39–47.

Xu J, Su X, Lim S, Griffin J, Carey E, Katz B, Tomich J, Smith JS, Wang W. Characterisation and stability of anthocyanins in purple-fleshed sweet potato P40. Food Chem. 2015;186: 90–96.

Singleton VL, Rossi JA. Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. Am J. Enol Vitic. 1965;16:144–158.

Wang LS, Stoner GD. Anthocyanins and their role in cancer prevention. Cancer Lett. 2008;269(2):281–290.

Lee MJ, Park JS, Choi DS, Jung MY. Characterization and quantitation of anthocyanins in purple-fleshed sweet potatoes cultivated in Korea by HPLC-DAD and HPLC-ESI-QTOF-MS/MS. J. Agric. Food Chem. 2013;61(12):3148–3158.

Fang ZX, Bhandari B. Effect of spray drying and storage on the stability of bayberry polyphenols. Food Chem. 2011; 129:1139–1147.