Storage Stability of Probiotic Soy Yoghurts with Enzyme Hydrolyzed African Breadfruit and Rice Additives
European Journal of Nutrition & Food Safety,
Aims: This study investigated the effect of culture type and storage time on pH, titratable acidity (%Lactic acid), viscosity and syneresis, probiotic viability and sensory properties of probiotic soy yoghurts with enzyme hydrolyzed African bread fruit (HABF) and rice syrup.
Methodology: Three sets of Soy-HABF Yoghurt were formulated by supplementing soymilk with 4% HABF and the addition of enzyme hydrolyzed rice syrup. The formulations were inoculated separately with Bifidiobacterium bifidum and Lactobacillus acidophillus as mono- and co-cultures and fermented at 42 °C for 8 h. Using standard methods, the samples were analyzed after fermentation representing day 1 and on the 5th, 10th, 15th and 20th days of storage at 4⁰ C.
Results: Culture type and the storage time had significant (P≤0.05) effect on physicochemical, probiotic count and sensory properties of the probiotic soy-HABF yoghurt. pH decreased significantly (P ≤ 0.05) with storage time from 4.36 - 4.06, 4.30 – 3.97 and 4.35 - 4.26 for B, L and BL (B. bifidum, L. acidophilus and the co-culture of B. bifidum, and L. acidophilus fermented samples). L. acidophilus had highest pH decrease. Titratable acidity, viscosity and syneresis index increased significantly (P≤0.05) with increase in storage time. Probiotic counts varied from 7.72-6.54 and 8.56 – 7.70 Log CFU/ml for B. bifidum and L. acidophilus respectively in the mono-culture samples B and L and from 7.66 – 5.65 and 7.90 – 6.57 Log CFU/ml for B.bifidum and L. acidophilus in sample BL. Assessors’ degree of likeness for the aroma, appearance, taste and texture of the probiotic soy-HABF yoghurt varied from neither like nor dislike to moderate likeness. The overall acceptability indicated that the L. acidophilus fermented product (sample L) was more acceptable to the assessors within the first 5 days of storage.
Conclusion: This study have shown that B.bifidum and L.acidophillus retained a viability of > log 6 within 15 days of storage. The physicochemical and sensory characteristics of soy/HABF yoghurt were also at optimum within the first 5 days at 4⁰ C.
- Probiotic viability
- soy yoghurt
- African breadfruit
- storage stability
- L acidophillus
- B. bifidum
How to Cite
Al-Saleh AA, Metwalli AA, Ismail EA. Physicochemical properties of Probiotic Frozen Yoghurt made from Camel Milk. Int J Dairy Technol. 2011;64(4):557–562.
Mongkontanawat N, Wongekalak L, Nonmuang W,Thumrongchote D. Yoghurt Production from Germinated Native Black Rice(Maepayatong Dum Rice) Inter. Journ Agric Technol. 2018;14(4):543-558.
Vij S, Hati S, Yadav D. Biofunctionality of Probiotic Soy Yoghurt Food and Nutrition Sciences. 2011;2:502-509.
Boylston TD, Vinderola CG, Ghoddusi H, Reinheimer J. Incorporation of Bifidobacteria into cheeses: Challenges and rewards. Int Dairy J. 2004;14(5):375-387.
Gibson GR, Probert HM, van Loo JAE, Rastall RA, Roberfroid MB. Dietary modulation of the human colonic microbiota: updating the concept of prebiotics. Nutri Res Reviews. 2004;17: 259-275.
Sadeq HA, Ismail A, Yazid MAM Mustafa S, Rokiah MY, Fouad, AH. () Prebiotics as functional foods: A review. J Fun Foods. 2013; 5:1542–1553.
Zare F, Champagne CP, Simpson BK, Orsat V, Boye JI. Effect of the addition of pulse ingredients to milk on acid production by probiotic and yoghurt starter cultures. LWT Food Sci Technol 2012; 45(2):155-160.
Agil R, Gaget A, Giliwa J, Hosseinian F. Lentils enhance probiotic growth in yoghurt and provide added benefit of antioxidant protection. LWT-Food Sci Technol. 2013; 50(1):45-49.
Boudjou S, Zaidi F, Hosseinian F, Oomah BD. Effect of Faba Bean (Vicia faba L.) flour on viability of probiotic bacteria during Kefir storage. J Food Res. 2014;3(6):13-22.
Lorusso IA, Rossana C, Montemurro M, Carlo GR. Use of Selected Lactic Acid Bacteria and Quinoa Flour for Manufacturing Novel Yogurt-Like Beverages. Foods. 2018;7:51(1- 20).
Barber LI, Osuji CM, Onuegbu NC ,Ogueke CC Quality Characteristics of Probiotic Soy Yoghurts with Enzyme Hydrolyzed African Breadfruit and Rice Additives.Am Journ of Food Sci and Technol, 2020;8;(6):233-241.
Osuji CM, Nwosu JC. Effect of Soybean Sprouting and Beta glucanase treatment of Wet Milled Soybeans on the Chemical Property of Soymilk from Different Variety of Soybeans. Nigerian Food J. 2011;29:(1) 94–102.
Champagne CP, Green-Johnson J, Raymond Y, Barrette J, Buckley N. Selection of probiotic bacteria for the fermentation of a soy beverage in combination with Streptococcus thermophilus. Food Res Int. 2009; 42:612–621.
Unal E, Akalin AS. Antioxidant and angiotensin-converting enzyme inhibitory activity of yoghurt fortified with sodium calcium caseinate or whey protein concentrate. Dairy Sci Technol. 2012; 92(6):627-639. DOI: 10.1007/s13594-012-0082-5
Iwe MO. The Science and Technology of Soybean. Rejoint Communication Services Ltd. 2003; 145.
Chou CC, Hou JW. Growth of Bifidobacteria in soymilk and their survival in the fermented drink during storage. Int J Food Microbiol. 2000; 56:113 – 121.
Donkor ON, Nilmini SLI, Stolic P, Vasiljevic T, Shah NP. Survival and activity of selected probiotic organism in set-type yoghurt during cold storage. Int Dairy J. 2007; 17:657-665.
Dave RI, Shah NP. Evaluation of Media for Selective Enumeration of Streptococcus thermophilus, Lactobacillus delbrueckii spp bulgaricus, Lactobacillus acidophilus and Bifidobacterium. J Dairy Sci, 1996;79: 1529 – 39.
Estevez AM, Mejia J, Figuerola F, Escobar B. Effect of Solid Content and Sugar Combinations on the Quality of Soymilk Based Yoghurt. J Food Process Preserv. 2010;34:87-97.
Wang J, Guo Z, Zhang Q, Yan L, Chen W X.-M, Liu X-M, Zhang H-P. Fermentation characteristics and transit tolerance of probiotic Lactobacillus casei Zhang in soymilk and bovine milk during storage. J Dairy Sci. 2008;92:92:2468–2476.
Farnworth ER, Mainville I, Desjardins MP, Gardner N, Fliss I, Champagne C. Growth of probiotic bacteria and bifidobacteria in soy yoghurt formulation. Int J Microbial. 2007; 116(1):174-81.
Athar IH, Shah MA, Khan UN. Effect of Various Stabilizers on Whey Separation (Syneresis) and Quality of Yoghurt. Pakistan Journal of Biological Sciences. 2000;3:1336-1338.
Martensson O, Oste R, Holst O. The eﬀect of yoghurt culture on the survival of probiotic bacteria in oat-based, non-dairy products. Food Res Int, 2002;35:775–784.
Scalabrini P, Rossi M, Spettoli P, Matteuzzi D. Characterization of Biﬁdoba cterium strains for use in soymilk ferment ation. Int J Food Microbiol, 1998;39:213–219.
Martínez-Villaluenga C, Frías J, Gómez R, Vidal-Valverde C. Influence of addition of raffinose family oligosaccharides on probiotic survival in fermented milk during refrigerated storage. Int Dairy J. 2006; 7:768-774.
Pinthong R, Macrae R, Rothwell J. The Development of Soya Based Yoghurt 1: Acid Production by Lactic Acid Bacteria. J Food Technol. 1980;5:647-652.
Cliff MA, Fan L, Sanford K, Stanich K, Doucette C, Raymond N. Descriptive Analysis and Early Stage Consumer Acceptance of Yogurts Fermented with Carrot Juice. J Dairy Sci. 2013;96:4160– 4172.
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